Institutes for pharmaceutical chemistry -
Prof. Dr. Michael Karas

 

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Expertise

  • Identification and characterization of proteins expressed in cells or cell compartments
  • Mass spectrometry (MALDI and ESI) of intact proteins, peptides, oligosaccharides, drugs and metabolites
  • Method development for investigation of membrane proteins and membrane protein   complexes
  • In-vitro assay for transcription/translation for search for new inhibitors of the bacterial   protein synthesis (antibiotics)
  • Method development in drug analysis
  • Liquid chromatography
  • Gel electrophoresis

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Resources and Abilities

  • 1D/2D-Gelelectrophoresis
  • Blue-native Gel electrophoresis
  • MALDI TOF Mass spectrometry
  • MALDI TOF-TOF Mass spectrometry
  • Nano-ESI-MS
  • HPLC-ESI-MS/MS
  • µ-bore, capillary- and nano-HPLC
  • TAP Tandem affinity purification for analysis of functional multi-protein complexes

 

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Publications

  • F. Hillenkamp, M. Karas, R.C. Beavis, B.T. Chait, Matrix- Assisted Laser Desorption/Ionization Mass Spectrometry of Biopolymers. Anal. Chem. 63 (1991) 1193A -1203A.

  • U. Bahr, M. Karas, Differentiation of "Isobaric" Peptides and Human Milk Oligosaccharides by Exact Mass Measurements Using ESI-orthogonal TOF Analysis. Rapid Commun. Mass Spectrom. 13 (1999) 1052-1058.

  • B. Finke, B. Stahl, A. Pfenninger, M. Karas, H. Daniel, G. Sawatzki, Analysis of High-Molecular-Weight Oligosaccharides from Human Milk by Liquid Chromatography and MALDI-MS. Anal. Chem. 71 (1999) 3755-3762.

  • M.Abdel Tawab, U. Bahr, B. Danieli, S. Gebhardt, M. Karas, S. Riva, M. Schubert-Zsilavecz, Electrospray Mass Spectrometry with Consecutive Fragmentation Steps (ES-MSn) as a Tool for Rapid and Sensitive Analysis of Ginsenosides and their Galactosyl Derivatives. Helv. Chim. Acta 83 (2000) 739-747.

  • A. Schmidt, U. Bahr, M. Karas, The Influence of Pressure in the First Pumping Stage on Analyte Desolvation and Fragmentation in Nano-ESI MS. Anal. Chem. 73 (2001) 6040-6046.

  • A. Schmidt, M. Karas, The influence of Electrostatic Interactions on the Detection of Heme-Globin Complexes in ESI-MS J. Am. Soc. Mass Spectrom. 12 (2001) 1092-1098.

  • A. Pfenninger, M. Karas, B. Finke, B. Stahl, Structural Analysis of Underivatized Neutral Human-Milk Oligosaccharides in the Negative Ion Mode by Nano-Electrospray MSn (Part 1 : Methodology). J. Am. Soc. Mass Spectrom. 13 (2002) 1331-1340.

  • A. Pfenninger, M. Karas, B. Finke, B. Stahl, Structural Analysis of Underivatized Neutral Human-Milk Oligosaccharides in the Negative Ion Mode by Nano-Electrospray MSn (Part 2 : Application to Isomeric Mixtures.) J. Am. Soc. Mass Spectrom. 13 (2002) 1341-48.

  • A. Schmidt, T. Dülks, M. Karas, Effect of different solution flow rate on analyte ion signals in nano-ESI MS, or : When does ESI turn to Nano-ESI. J. Am. Soc. Mass Spectrom. 14 (2003) 492-500.

  • R. Krüger, M. Karas, Ion Formation in MALDI: The Cluster Ionization Mechanism. Chem. Rev. 103 (2003) 427-439.

  • C. Corvey, T. Stein, S. Düsterhus, M. Karas, K.-D. Entian, Activation of subtilin precursors by Bacillus subtilis extracellular serine proteases subtilisin (AprE), WprA, and Vpr. Biochem. Biophys. Res. Commun. 304 (2003) 48-54.

  • K. Ekroos, C. S. Ejsing, U. Bahr, M. Karas, K. Simons, A. Shevchenko. Charting molecular composition of phosphatidylcholines by fatty acid scanning and ion trap MS3 fragmentation. J. Lipid Res. 44 (2003) 2181-2192.

  • J.-H. Keller, M. Karas, W.E. Müller, D.A. Volmer, G.P. Eckert, M. Abdel-Tawab, H.H. Blume, T. Dingermann, M. Schubert-Zsilavecz, Determination of Hyperforin in Mouse Brain by Rapid High Performance Liquid chromatography/Tandem Mass Spectrometry. Anal. Chem. 75 (2003) 6084-6088.

 

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Staff:

  • Dr. Ute Bahr (Protein MS, Oligosaccharide MS)
  • Dipl. Ing. Tobias Beckhaus (Proteomics)
  • Dipl. biologist Carsten Corvey (Proteomics, TAP)
  • Dipl. chemist Marianne Gallardo (Proteomics)
  • Dipl. food chemist Sandra Hofmann (Proteomics, HPLC MS)
  • Pharmacist Martin Hample (Proteomics)
  • Pharmacist Tri Hoang (Proteomics)
  • Pharmacist Björn Meyer (Proteomics, Membrane proteins)
  • Pharmacist Isam Rais (Proteomics, Membrane proteins)
  • Dipl. Ing. Eric Schulz (Proteomics)
  • Dipl. chemist Corina Hunger (RNA/DNA MS)
  • Pharmacist Markus Weidlich (rRNA Inhibitors)
  • Pharmacist Frank Dannenberg (rRNA Inhibitors)

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ZAFES-Coordinator:

 

 

 

INSTITUTES AND
MEDICAL CENTERS

 
 

RHEUMATOLOGY

 
 

GASTROENTEROLOGY - HEPATOLOGY

 
 

PAEDIATRIC HAEMATOL., ONCOL. & HAEMOSTASEOLOGY

 
 

NEURORADIOLOGY

 
 

CLINICAL PHARMACOLOGY

 
 

GENERAL PHARMACOLOGY &
TOXICOLOGY

 
 

IMMUNE PHARMACOLOGY

 
 

INTERDISCIPLINARY PHARMACOLOGY

 
 

PHARMACEUTICAL TECHNOLOGY

 

PHARMACEUTICAL CHEMISTRY

 

PROF. KARAS
PROF. SCHUBERT-ZSILAVECZ
PROF. STARK
PROF. STEINHILBER

 

 

 

 

 

PHARMACEUTICAL
BIOLOGY

 
 

GEORG SPEYER HAUS

 
 

ORGANIC CHEMISTRY &
CHEMICAL BIOLOGY

 
 

NEUROLOGY

 
 

ANESTHESIOLOGY, INTENSIVE CARE AND
PAIN CONTROL

 
 

OBSTETRICS AND GYNECOLOGY

 
 

DEPARTMENT OF DERMATOLOGY & VENEROLOGY

 
 

CARDIOVASCULAR PHYSIOLOGY

 
 

PEDIATRIC PNEUMOLOGY, ALLERGY AND CYSTIC FIBROSIS

MEDICAL VIROLOGY

ECOLOGY, EVOLUTION AND DIVERSITY, DEPARTMENT AQUATIC ECOTOXICOLOGY

 
 

ORAL, CRANIO-
MAXILLOFACIAL AND
FACIAL PLASTIC SURGERY

 
 

GENERAL AND VASCULAR SURGERY

 
 

CLINICAL NEUROANATOMY

 

GENERAL PEDIATRICS

HAEMATOLOGY, ONCOLOGY

DIAGNOSTIC AND INTERVENTIONAL RADIOLOGY